Normal breast epithelium

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Estrogen exposure is widely believed to contribute to breast cancer BC risk through the induction of proliferation. We studied the rates of breast epithelial cell proliferation and apoptosis in pre, peri, and postmenopausal women. Archival paraffin embedded sections of normal epithelium were chosen from women undergoing breast surgery.

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Metrics details. Our efforts to prevent and treat breast cancer are significantly impeded by a lack of knowledge of the biology and developmental genetics of the normal mammary gland. In order to provide the specimens that will facilitate such an understanding, The Susan G.

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The breast consists of three main components, the skin, the subcutaneous adipose tissue and the functional glandular tissue that comprises both parenchyma and stroma. The collecting ducts open at the nipple through which the infant obtains milk during breastfeeding. Below the nipple, the collecting ducts dilate to form the lactiferous sinuses.

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Metrics details. Enhancer of zeste homolog 2 EZH2 is a polycomb-group protein that is involved in stem cell renewal and carcinogenesis. In breast cancer, increased EZH2 expression is associated with aggressiveness and has been suggested to identify normal breast epithelium at increased risk of breast cancer development. However, the association between EZH2 expression in benign breast tissue and breast cancer risk has not previously been evaluated in a large prospective cohort.

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Breast epithelial response to estradiol may play an important role in breast cancer etiology. We have examined the relationship between serum estradiol and progesterone levels and normal breast epithelial expression of estrogen receptor ER alpha, progesterone receptor PgRand epithelial proliferation as reflected by the Ki labeling index in women 50 newly diagnosed breast cancer cases and 71 benign breast disease controls. Simultaneous samples of grossly normal breast tissue and venous blood were obtained from women undergoing breast surgery.

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Cancer is a heterogeneous and tissue-specific disease. Thus, the tissue of origin reflects on the natural history of the disease and dictates the therapeutic approach. It is suggested that tissue differentiation, mediated mostly by epigenetic modifications, could guide tissue-specific susceptibility and protective mechanisms against cancer.

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A complete solution to propagate mammary epithelial cells with excellent morphology and proliferation in serum-free conditions. Biosafety classification is based on U. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

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